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Literature summary extracted from

  • Grauffel, C.; Yang, B.; He, T.; Roberts, M.F.; Gershenson, A.; Reuter, N.
    Cation-pi interactions as lipid-specific anchors for phosphatidylinositol-specific phospholipase C (2013), J. Am. Chem. Soc., 135, 5740-5750.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.13 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Bacillus thuringiensis

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.13 Y246A the variant shows significant membrane binding defects Bacillus thuringiensis
4.6.1.13 Y246A/Y247A the variant shows significant membrane binding defects Bacillus thuringiensis
4.6.1.13 Y247A the variant shows significant membrane binding defects Bacillus thuringiensis
4.6.1.13 Y86A/Y88A the mutations decrease membrane affinity for the enzyme Bacillus thuringiensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.6.1.13 membrane
-
 Bacillus thuringiensis 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.13 34800
-
 x * 34800, calculated from amino acid sequence Bacillus thuringiensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.13 1-phosphatidyl-1D-myo-inositol Bacillus thuringiensis
-
 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol
-
 ?
4.6.1.13 1-phosphatidyl-1D-myo-inositol Bacillus thuringiensis N168C
-
 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.13 Bacillus thuringiensis P08954
-
-
4.6.1.13 Bacillus thuringiensis N168C P08954
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.13 Q-Sepharose column chromatography and phenyl Sepharose column chromatography Bacillus thuringiensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.13 1-phosphatidyl-1D-myo-inositol
-
 Bacillus thuringiensis 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol
-
 ?
4.6.1.13 1-phosphatidyl-1D-myo-inositol
-
 Bacillus thuringiensis N168C 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol
-
 ?

Subunits

EC Number Subunits Comment Organism
4.6.1.13 ? x * 34800, calculated from amino acid sequence Bacillus thuringiensis

Synonyms

EC Number Synonyms Comment Organism
4.6.1.13 phosphatidylinositol-specific phospholipase C
-
 Bacillus thuringiensis
4.6.1.13 PI-PLC
-
 Bacillus thuringiensis

General Information

EC Number General Information Comment Organism
4.6.1.13 physiological function the enzyme is a virulence factor Bacillus thuringiensis